This detailed volume explores the notable progress in the field of zinc finger proteins (ZFP) study through widely used methods and prools involving their biological functions and applications. Beginning with a section on the basic biology of ZFPs and design and applications of custom ZFPs, the book continues by covering methods for the evaluation and prevention of ZFNmediated cytotoxicity as well as a collection of the representative methods of ZFN delivery. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, stepbystep, readily reproducible laboratory prools, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Zinc Finger Proteins: Methods and Prools aims to prompt both the understanding of ZFP biology and the development of nextgeneration ZFP therapeutics.
This detailed volume explores the notable progress in the field of zinc finger proteins (ZFP) study through widely used methods and prools involving their biological functions and applications. Beginning with a section on the basic biology of ZFPs and design and applications of custom ZFPs, the book continues by covering methods for the evaluation and prevention of ZFNmediated cytotoxicity as well as a collection of the representative methods of ZFN delivery. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, stepbystep, readily reproducible laboratory prools, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Zinc Finger Proteins: Methods and Prools aims to prompt both the understanding of ZFP biology and the development of nextgeneration ZFP therapeutics.
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