This book details recently developed technologies and conventionally employed cytological proceduresfor the study of XChromosome Inactivation. Chapters detail live imaging, bioinformatic methods, fluorescence in situ hybridization, and immunofluorescence, and procedures to optimize the study of molecular mechanism underlying X chromosome inactivation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, stepbystep, readily reproducible laboratory prools, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cuttingedge, XChromosome Inactivation: Methods Prools aims to be useful for researchers in the field of epigenetics, chromatin, noncoding RNA, and nuclear architecture.
This book details recently developed technologies and conventionally employed cytological proceduresfor the study of XChromosome Inactivation. Chapters detail live imaging, bioinformatic methods, fluorescence in situ hybridization, and immunofluorescence, and procedures to optimize the study of molecular mechanism underlying X chromosome inactivation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, stepbystep, readily reproducible laboratory prools, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cuttingedge, XChromosome Inactivation: Methods Prools aims to be useful for researchers in the field of epigenetics, chromatin, noncoding RNA, and nuclear architecture.
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