This volume provides a comprehensive collection of current methods and prools to study posttranscriptional base modifications in RNA with special focus on methylation. The prools in this book discuss stateoftheart methods for investigating aspects of RNA methylation on different types of RNA. The prools cover topics such as wetlab techniques for the detection of methylation, instructions for bioinformatics analyses of transcriptomescale data, and prools for the functional examination of RNA modifications and enzymes. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, stepbystep, readily reproducible laboratory prools, and tips on troubleshooting and avoiding known pitfalls. Cuttingedge and thorough, RNA Methylation: Methods and Prools is a valuable resource for biochemists and molecular biologists, from various fields, who wish to investigate different types of RNA methylations.
This volume provides a comprehensive collection of current methods and prools to study posttranscriptional base modifications in RNA with special focus on methylation. The prools in this book discuss stateoftheart methods for investigating aspects of RNA methylation on different types of RNA. The prools cover topics such as wetlab techniques for the detection of methylation, instructions for bioinformatics analyses of transcriptomescale data, and prools for the functional examination of RNA modifications and enzymes. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, stepbystep, readily reproducible laboratory prools, and tips on troubleshooting and avoiding known pitfalls. Cuttingedge and thorough, RNA Methylation: Methods and Prools is a valuable resource for biochemists and molecular biologists, from various fields, who wish to investigate different types of RNA methylations.
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