Regulatory B Cells: Methods and Prools present the current experimental setups and methodologies useful for the identification and characterization of B cells with suppressive functions and for the study of their biological and immunological properties. Organized into four sections, this detailed volume covers the basic methods for the isolation and immunophenotypical characterization of specific B cell subsets from mouse and human tissues, methods for the investigation of the mechanisms of immune suppression operated by B cells, several experimental approaches for the ex vivo generation/expansion of IL10producing B cells, as well as procedures for the study of the immune suppressive function of B cells in specific pathological settings. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, stepbystep, readily reproducible laboratory prools and tips on troubleshooting and avoiding known pitfalls. Practical and authoritative, Regulatory B Cells: Methods and Prools serves as an ideal guide for immunologists as well as for cell and molecular biologists interested in the intricacies of B cell biology.
Regulatory B Cells: Methods and Prools present the current experimental setups and methodologies useful for the identification and characterization of B cells with suppressive functions and for the study of their biological and immunological properties. Organized into four sections, this detailed volume covers the basic methods for the isolation and immunophenotypical characterization of specific B cell subsets from mouse and human tissues, methods for the investigation of the mechanisms of immune suppression operated by B cells, several experimental approaches for the ex vivo generation/expansion of IL10producing B cells, as well as procedures for the study of the immune suppressive function of B cells in specific pathological settings. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, stepbystep, readily reproducible laboratory prools and tips on troubleshooting and avoiding known pitfalls. Practical and authoritative, Regulatory B Cells: Methods and Prools serves as an ideal guide for immunologists as well as for cell and molecular biologists interested in the intricacies of B cell biology.
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