This volume presents established bioinformatics tools and databases for function prediction of proteins. Reflecting the diversity of this active field in bioinformatics, the chapters in this book discuss a variety of tools and resources such as sequence—, structure—, systems—, and interaction—based function prediction methods, tools for functional analysis of metagenomics data, detecting moonlighting—proteins, sub—cellular localization prediction, and pathway and comparative genomics databases. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, step—by—step instructions of how to use software and web resources, use cases, and tips on troubleshooting and avoiding known pitfalls. Thorough and cutting—edge, Protein Function Prediction: Methods and Prools is a valuable and practical guide for using bioinformatics tools for investigating protein function