This booklet is the seventh volume of the Dattoli Cancer Foundation's Prostate Cancer Essentials for Survival series. It is a guide for informed patients, especially those who are confronted with the prospect of a prostate biopsy. The booklet offers a comprehensive overview of the ultrasound-guided prostate biopsy and related laboratory tests, including the prostate specific antigen (PSA) blood test. Two leading cancer care professionals specializing in state-of-the-art radiotherapy guide readers step by step through questions to be considered before undergoing a prostate biopsy. The authors share their wealth of knowledge and their experience with the Dattoli Cancer Center & Brachytherapy Research Institute, which has the largest combined program in the country for brachytherapy and Intensity Modulated Radiation Therapy (IMRT), specializing in Dynamic Adaptive Radiation Therapy (DART), the most advanced form of radiotherapy currently available.