Modification of target protein properties by reversible phosphorylation events has been found to be one of the most prominent cellular control processes in all organisms. Recent advances in the areas of molecular biology and biochemistry are presenting new possibilities for reaching an unprecedented depth and a proteomewide understanding of phosphorylation processes in plants as well as in other species. The major goal of Plant Kinases: Methods and Prools is to provide the experimentalist with a detailed account of the practical steps necessary for successfully carrying out each prool in his or her own laboratory. Plant protein kinases specifically addressed in this volume are members of the plant MAP kinase cascade, cyclin and Calciumdependent protein kinases, and plant sensor and receptor kinases. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, stepbystep, readily reproducible laboratory prools, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Plant Kinases: Methods and Prools will prove a useful laboratory companion to both novice and seasoned researchers by facilitating the practical work that will lead them to new and exciting insights in this dynamic field.
Modification of target protein properties by reversible phosphorylation events has been found to be one of the most prominent cellular control processes in all organisms. Recent advances in the areas of molecular biology and biochemistry are presenting new possibilities for reaching an unprecedented depth and a proteomewide understanding of phosphorylation processes in plants as well as in other species. The major goal of Plant Kinases: Methods and Prools is to provide the experimentalist with a detailed account of the practical steps necessary for successfully carrying out each prool in his or her own laboratory. Plant protein kinases specifically addressed in this volume are members of the plant MAP kinase cascade, cyclin and Calciumdependent protein kinases, and plant sensor and receptor kinases. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, stepbystep, readily reproducible laboratory prools, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Plant Kinases: Methods and Prools will prove a useful laboratory companion to both novice and seasoned researchers by facilitating the practical work that will lead them to new and exciting insights in this dynamic field.
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