This volume details experimental approaches used to investigate phagocytosis and phagosome maturation. Chapters present methods and prools on quantifying uptake and phagosome maturation using biophysical and biochemical approaches, proteomics, microscopy, and flow cytometry. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, stepbystep, readily reproducible laboratory prools, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cuttingedge, Phagocytosis and Phagosomes: Methods and Prools aims to be an important resource for both experts in the field and for those investigators delving into phagocytosis and phagosome maturation for the first time.
This volume details experimental approaches used to investigate phagocytosis and phagosome maturation. Chapters present methods and prools on quantifying uptake and phagosome maturation using biophysical and biochemical approaches, proteomics, microscopy, and flow cytometry. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, stepbystep, readily reproducible laboratory prools, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cuttingedge, Phagocytosis and Phagosomes: Methods and Prools aims to be an important resource for both experts in the field and for those investigators delving into phagocytosis and phagosome maturation for the first time.
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