These proceedings are a record of the Multiple Classifier Systems Workshop, MCS 2007, held at the Institute of Information Theory and Automation, Czech Academy of Sciences, Prague in May 2007. Being the seventh in a well-established series of meetings providing an international forum for the discussion of issues in multiple classifier system design, the workshop achieved its objective of bringing together researchers from diverse communities (neural networks, pattern rec- nition, machine learning and statistics) concerned with this research topic. From more than 80 submissions, the Programme Committee selected 49 - pers to create an interesting scientific programme. The special focus of MCS 2007 was on the application of multiple classifier systems in biometrics. This part- ular application area exercises all aspects of multiple classifier fusion, from - tramodal classifier combination, through confidence-based fusion, to multimodal biometric systems. The sponsorship of MCS 2007 by the European Union N- work of Excellence in Biometrics BioSecure and in Multimedia Understanding through Semantics, Computation and Learning MUSCLE and their assistance in selecting the contributions to the MCS 2007 programme consistent with this theme is gratefully acknowledged.