This book focuses on the importance of omics strategies and de-replication analysis to unveil new molecules from microbial sources with diverse chemical structures and biological functions. Chapters address metabolomics strategies, which will lead to a better understanding of the chemical interactions between microorganisms, plant-microorganisms, and virus-microorganisms. Authors also describe analytical tools used in microbial metabolomics and natural products discovery, in addition to describing a step-by-step prool to identify and annotate metabolites using various databases and online platforms. The book presents the newest research, tools, and prools for chemists, biochemists, bio-and chemical engineers, and biotechnologists, among others.