This self-contained textbook, now in a thoroughly revised and expanded second edition, takes a matrix-oriented approach to Linear Algebra. It presents a complete theory, including all details and proofs, culminating in the Jordan canonical form and its derivation. Throughout, the book emphasizes the practical applicability of results. It therefore also covers special topics in Applied Linear Algebra, such as matrix functions, the singular value decomposition, the Kronecker product, and linear matrix equations. New to this edition are topics such as the Frobenius canonical form and a more detailed treatment of infinite-dimensional vector spaces, along with many additional exercises. The book’s matrix-oriented approach enhances intuition and simplifies abstract concepts, making them easier to understand and to apply in real-world scenarios. Key applications are illustrated through detailed examples. Additionally, several "MATLAB Minutes" allow students to explore concepts and results through computational experiments, supported by a brief introduction to MATLAB fundamentals. Together with over 380 exercises, this encourages active engagement with the material.