This detailed volume presents a series of prools dealing with different aspects of inclusion body (IB) processing, from cloning procedures to purification of refolded product. Commencing with chapters on upstream processing, looking into different expression strategies for IB production, the book continues with downstream applications, highlighting early protein purification and subsequent analytics, as well as success stories of IBbased processes. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, stepbystep and readily reproducible laboratory prools, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Inclusion Bodies: Methods and Prools serves as an ideal resource for facilitating diverse aspects of IB processing.
This detailed volume presents a series of prools dealing with different aspects of inclusion body (IB) processing, from cloning procedures to purification of refolded product. Commencing with chapters on upstream processing, looking into different expression strategies for IB production, the book continues with downstream applications, highlighting early protein purification and subsequent analytics, as well as success stories of IBbased processes. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, stepbystep and readily reproducible laboratory prools, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Inclusion Bodies: Methods and Prools serves as an ideal resource for facilitating diverse aspects of IB processing.
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