This book details the development of methods and models to study the HIV1 viral reservoir with the ultimate goal of achieving a functional cure of HIV infection. Chapters are divided into six parts covering cell lines, in vitro and ex vivo primary cell models of persistent infection, in vitro and ex vivo tissuederived models, infected animal models human immune cells, methods of detection and analysis of the reservoir, and current approaches to achieve either a functional cure or cARTfree longterm remission. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and stepbystep, readily reproducible prools. Authoritative and cuttingedge, HIV Reservoirs: Methods and Prools provides a comprehensive, updated collection of stateofart methodologies and models to tackle the HIV1 viral reservoir.
This book details the development of methods and models to study the HIV1 viral reservoir with the ultimate goal of achieving a functional cure of HIV infection. Chapters are divided into six parts covering cell lines, in vitro and ex vivo primary cell models of persistent infection, in vitro and ex vivo tissuederived models, infected animal models human immune cells, methods of detection and analysis of the reservoir, and current approaches to achieve either a functional cure or cARTfree longterm remission. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and stepbystep, readily reproducible prools. Authoritative and cuttingedge, HIV Reservoirs: Methods and Prools provides a comprehensive, updated collection of stateofart methodologies and models to tackle the HIV1 viral reservoir.
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