This second edition provides new and updated prools that can be used for generation of knockout animals. Chapters guide the reader through basic prools for three genome editing technologies, target design tools, and specific prools for each animal. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible prools, and notes on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Genome Editing in Animals: Methods and Prools, Second Edition aims to be a useful practical guide to researches to help further their study in this field.