Remarkably, while G proteincoupled receptors (GPCRs) are highly prevalent in animals and yeast, very few candidate GPCRs have been identified in plants. In G ProteinCoupled Receptor Signaling in Plants: Methods and Prools, experts in the field describe techniques used in the study of small GTPases and related proteins. Beginning with a chapter on bioinformatics approaches for GPCR discovery, this detailed volume continues with chapters on heterotrimeric G protein subunits, RabGTPases, as well as lipid modifications, including myristoylation, acylation, and prenylation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, stepbystep, readily reproducible laboratory prools, and tips on troubleshooting and avoiding known pitfalls. Practical and dependable, G ProteinCoupled Receptor Signaling in Plants: Methods and Prools aims to aid further studies into the roles of small GTPases which will help elucidate numerous key processes in plants.
Remarkably, while G proteincoupled receptors (GPCRs) are highly prevalent in animals and yeast, very few candidate GPCRs have been identified in plants. In G ProteinCoupled Receptor Signaling in Plants: Methods and Prools, experts in the field describe techniques used in the study of small GTPases and related proteins. Beginning with a chapter on bioinformatics approaches for GPCR discovery, this detailed volume continues with chapters on heterotrimeric G protein subunits, RabGTPases, as well as lipid modifications, including myristoylation, acylation, and prenylation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, stepbystep, readily reproducible laboratory prools, and tips on troubleshooting and avoiding known pitfalls. Practical and dependable, G ProteinCoupled Receptor Signaling in Plants: Methods and Prools aims to aid further studies into the roles of small GTPases which will help elucidate numerous key processes in plants.
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