This volume looks at the evolution of the topo field over the last year and discusses how DNA topoisomerases are directly and indirectly involved in numerous cellular processes. The chapters in this book cover various topics, such as type 1 topos with an evolutionary perspective; antimicrobial agents targeting topos; experimental prools related to DNA topology, including a basic technique to measure DNA supercoiling in bacteria; prools for in vitro studies, including assays to detect RNA topo activity; topo inhibitors; and a study of plasmidmediated quinolone resistance in bacteria. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, stepbystep, readily reproducible laboratory prools, and tips on troubleshooting and avoiding known pitfalls. Cuttingedge and comprehensive, DNA Topoisomerases: Methods and Pro ols is intended for specialists and nonspecialists with an overview of selected hot topics in the field, and the latest prools to study DNA topology, topoisomerases functions and inhibition by various compounds.
This volume looks at the evolution of the topo field over the last year and discusses how DNA topoisomerases are directly and indirectly involved in numerous cellular processes. The chapters in this book cover various topics, such as type 1 topos with an evolutionary perspective; antimicrobial agents targeting topos; experimental prools related to DNA topology, including a basic technique to measure DNA supercoiling in bacteria; prools for in vitro studies, including assays to detect RNA topo activity; topo inhibitors; and a study of plasmidmediated quinolone resistance in bacteria. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, stepbystep, readily reproducible laboratory prools, and tips on troubleshooting and avoiding known pitfalls. Cuttingedge and comprehensive, DNA Topoisomerases: Methods and Pro ols is intended for specialists and nonspecialists with an overview of selected hot topics in the field, and the latest prools to study DNA topology, topoisomerases functions and inhibition by various compounds.
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