The last twenty years have seen significant strides in radiosurgical care, much of which was made possible by the CyberKnife technology. With an ever-increasing number of CyberKnife stereotactic radiosurgery units installed across the globe and thousands of physicians using them on a daily basis, the field of CyberKnife technology has grown rapidly. As minimally invasive, frameless, stereotactic radiosurgery gains popularity, this formal reference will soon become indispensable in understanding the application of CyberKnife SRS. This text provides a comprehensive reference for the treatment of various pathologies; it is meant to serve as manual for both new and experienced clinicians alike. Some of the most experienced CyberKnife users from around the world have contributed their knowledge and experience using CyberKnife. Each chapter covers a different disease process, focusing on various spinal pathologies. The content specifically highlights how the current use of CyberKnife relates to other radiosurgical modalities and treatment options by discussing advantages, disadvantages, dosing, complications, and efficacy. We hope that this text will serve as a reference, guiding clinicians in the application of an emerging technology.