This volume focuses on monoADPribosylation and enzymes that use NAD+ including Sirtuins, PARPs, and bacterial and eukaryotic ADPribosyltransferases. The chapters in this book are organized into eight parts, and offer detailed descriptions of key prools used to study topics such as in vitro techniques for ADPribosylation substrate identification; biochemical and biophysical assays of PARWWE domain interactions; monitoring expression and enzyme activity of ectoARTCs; HPLCbased enzymes assays for Sirtuins; and identifying target RNAs of PARPs. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, stepbystep, readily reproducible laboratory prools, and tips on troubleshooting and avoiding known pitfalls. Cuttingedge and thorough, ADPribosylation and NAD+ Utilizing Enzymes: Methods and Prools is a valuable resource for anyone interested in this developing and expanding field.
This volume focuses on monoADPribosylation and enzymes that use NAD+ including Sirtuins, PARPs, and bacterial and eukaryotic ADPribosyltransferases. The chapters in this book are organized into eight parts, and offer detailed descriptions of key prools used to study topics such as in vitro techniques for ADPribosylation substrate identification; biochemical and biophysical assays of PARWWE domain interactions; monitoring expression and enzyme activity of ectoARTCs; HPLCbased enzymes assays for Sirtuins; and identifying target RNAs of PARPs. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, stepbystep, readily reproducible laboratory prools, and tips on troubleshooting and avoiding known pitfalls. Cuttingedge and thorough, ADPribosylation and NAD+ Utilizing Enzymes: Methods and Prools is a valuable resource for anyone interested in this developing and expanding field.
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